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Cisplatin assay

After observing the enhanced cell-killing efficacy of the combination of CDDP and BV10 in various cancer cell lines and studying cell death processes, we further investigated the anti-cancer effect of this combination in the xenograft mouse models of lung, ovarian and cervical cancers. The typical dose of … See more The results of the effect of the combination of CDDP with BV10 on in vitro cell viability and clonogenic death are shown in Fig. 3. Figure 3A shows the … See more To demonstrate the DET mechanism of the combination, we made real-time measurements of the reaction between CDDP and BV10 … See more Figure 4A shows representative images of HeLa cells treated by CDDP with/without the combination with BV10 for 12 h, where apoptotic cells were … See more WebMar 1, 2024 · The developed and validated assay presented here is a highly sensitive LC-MS/MS method developed and validated for the quantitative determination of cisplatin following derivatization with diethyldithiocarbamate (DDTC) in order to detect platinum (Pt) of cisplatin, suitable for pharmacokinetic studies in rats and to further use it to study …

The changing 50% inhibitory concentration (IC50) of cisplatin

WebMar 1, 2024 · Cisplatin (cis-diamminedicholroplatinum II, Fig. 1) is a platinum complex bound to two chloride atoms and two ammonia molecules which makes it highly soluble … thinking shape png https://felder5.com

Development and validation of a LC-MS/MS assay for ... - PubMed

WebCisplatin is a key chemotherapeutic agent for the treatment of esophageal cancer. We examined the intracellular localization of cisplatin in esophageal cancer cell lines and determined their sensitivity to cisplatin using in-air micro-PIXE (particle induced X … WebOct 22, 2024 · Cisplatin is a commonly used anticancer drug, but nephrotoxicity is a dose-limiting adverse effect. Recent experimental and clinical observations have demonstrated that multiple injections of... WebThe MicroMolar Cisplatin Assay Kit (Catalog number CPT200) is designed for throughput measurement of micromolar concentrations of Cisplatin. The assay is based on the … thinking shadow

Development and validation of a LC–MS/MS assay for …

Category:Cellular responses to Cisplatin-induced DNA damage - PubMed

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Cisplatin assay

Effects of cisplatin on the proliferation, invasion and …

WebAug 6, 2015 · The chemotherapeutic use of cisplatin is limited by its severe side effects. In this study, by conducting different omics data analyses, we demonstrated that cisplatin … WebOct 25, 2016 · The MTT assay is currently the most extensively used method for IC50 measurements. Here, we dissected the critical reasons behind MTT-dependent IC50 …

Cisplatin assay

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WebMar 16, 2024 · The migratory and invasive abilities of BC cells treated with both siR‑β‑catenin and cisplatin were examined with Transwell assays. The CD44 antigen/intercellular adhesion molecule 1 expression ratio, cell … WebAug 8, 2010 · Cisplatin is one of the most effective anticancer agents widely used in the treatment of solid tumors. It is generally considered as a cytotoxic drug which kills cancer cells by damaging DNA and inhibiting DNA synthesis. How cells respond to cisplatin-induced DNA damage plays a critical role in deci …

WebApr 11, 2024 · Cisplatin is a widely used chemotherapeutic agent for treatment of various cancers. However, treatment with cisplatin is associated with drug resistance and … WebCisplatin is also sometimes used to treat head and neck cancer (including cancer of the mouth, lip, cheek, tongue, palate, throat, tonsils, and sinuses), lung cancer, cancer of the …

WebAdjuvant cisplatin, 70 mg every three weeks, was given, for a total of 1680 mg. In February 1979, while he was receiving cisplatin, fever developed, accompanied by dark urine, a … WebCisplatin is a chemotherapy drug that contains the metal platinum. It stops or slows growth of cancer cells and other cells that grow rapidly by damaging their DNA. FDA Approved …

WebJan 18, 2024 · Cisplatin was administered by intraperitoneal injection in mouse. DNA damage, excision repair, and gene expression were assayed by Damage-seq, XR-seq, and RNA-seq. b PCA reveals tissue-specific...

Web(A,B) MTT assay. The cisplatin-resistant cells and their parental cells were exposed to different concentrations of cisplatin for 72 h and viability was related to untreated … thinking shiftWebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … thinking shedWebAbstract Cisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. ... Cells … thinking should never submit to itselfWebMar 1, 2024 · Cisplatin is a platinum containing compound used for the treatment of different types of cancers. Quantitative determination of cisplatin has been carried out … thinking shapes alexander davisWebSep 15, 2024 · Cisplatin Injection (cisplatin injection (cisplatin (cisplatin injection) injection) ) is a sterile aqueous solution, available in 50, 100 and 200 mL multiple dose … thinking sheepWebElectron microscopywas used to observe ultrastructure changes in cell. Results: The depletion of reduced glutathione caused by cisplatin and the inactivation of glutathione peroxidase played the vital role in the underlying mechanism. thinking should become your capital assethttp://www.pharmacopeia.cn/v29240/usp29nf24s0_m17910.html thinking should