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Pan02 cell

WebThe PDAC cell line (PAN02 cells; DCTD Tumor Repository, NCI-Frederick, Frederick, MD) was cultured and expanded in Dulbecco’s modified Eagle’s medium (DMEM; Nacalai Tesque, Kyoto, Japan) supplemented with 10% heat-inactivated fetal bovine serum and 100 µg/mL penicillin and streptomycin (P/S; Life Technologies, Carlsbad, California, USA). WebSodium selenite acts by depleting enzymes that protect against cellular oxidative stress. To determine its effect alone or in combination with gemcitabine (GMZ) in pancreatic cancer, we used PANC-1 and Pan02 cell lines and C57BL mice bearing a Pan02-generated tumor. Our results demonstrated a significant inhibition of pancreatic cancer cell viability with the …

Establishment of an orthotopic pancreatic cancer mouse …

WebDec 8, 2016 · Here, we show that treatment of KPC and Pan02 murine PDAC cells with RT and gemcitabine upregulated PD-L1 expression in a JAK/Stat1-dependent manner. In … WebPan02 cells, treated with 0ng_per_ml for 4hours: Organism: Mus musculus: Characteristics: strain: C57BL/6 cell line: Pan02 cell type: pancreatic carcinoma: Treatment protocol: 100 ng/ml TWEAK or media control was added on day 2 to subconfluent cells. For each time point and treatment, five replicates were generated. One replicate from each ... sleep documentary bbc https://felder5.com

Tumor growth is reduced in mice injected with IL-6 Pan02

WebMouse Pan02 Pancreatic Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … WebDescription. The mouse Panc02 is a well-established mouse pancreatic epithelial cell line, derived from adenocarcinoma model developed by chemical induction with 3-MCA (3-methylcholanthrene) in male C57BL/6 mice.It is used as pancreatic cancer models for drug development and clinical research. Gentarget generated this GFP reporter cells, t … WebFeb 2, 2024 · Pancreatic ductal adenocarcinoma (PDAC), which covers more than 90% of pancreatic cancer cases and is known simply as pancreatic cancer, 1,2 is a highly … sleep downunder 2021 virtual platform

Cystine–glutamate antiporter xCT deficiency suppresses tumor ... - PNAS

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Pan02 cell

Pan_02 murine pancreatic cancer model - PubMed

WebFeb 14, 2012 · Pan02 cells have a mesenchymal-like phenotype. Fluorescence immunohistochemistry (A–B) and immunocytochemistry (C) were utilized to assess the epithelial versus mesenchymal nature of Pan02 cells. (A) Frozen tumor sections were stained with antibody rabbit anti E-Cadherin (red) to mark epithelial cells. DAPI (blue) … WebPan02 is a well-established mouse pancreatic ductal adenocarcinoma (PDAC) model developed by chemical induction with 3-methylcholanthrene (3-MCA) (1). The cell line …

Pan02 cell

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WebPan02-OVA cells were engineered by stable transduction of Pan02 cells with pChac-puro plasmid expressing full-length chicken ovalbumin (OVA; LakePharma). All cells were confirmed mycoplasma negative and identity of all cell lines was validated by short-tandem repeat profiling. CCR4 antagonist WebDescription. The mouse Panc02 is a well-established mouse pancreatic epithelial cell line, derived from adenocarcinoma model developed by chemical induction with 3-MCA (3 …

WebApr 12, 2024 · The claudin 18.2 (CLDN18.2) antigen is frequently expressed in malignant tumors, including pancreatic ductal adenocarcinoma (PDAC). Although CLDN18.2-targeted CAR-T cells demonstrated some therapeutic efficacy in PDAC patients, further improvement is needed. One of the major obstacles might be the abundant cancer-associated … WebNov 20, 2024 · ( a) Pancreatic stellate cells (PSCs) from wild type (wt), genetically engineered mice (GEM), and human pancreatic tissues are isolated either by density …

WebPan02: Murine pancreatic ductal adenocarcinoma. (The above parameters are from one study. For further information on this cell line and other parameters, including different strains, vendors, implant type and location and/or standards of care, please contact us.) ***Please note that cell lines are not for sale and unavailable for purchase.***. WebApr 24, 2024 · Both MC38 and Pan02 cell lines displayed dependence on xCT, as deletion of xCT rendered cells unable to proliferate in vitro (Fig. 1 H and I). Compared with their WT counterparts, xCT-deficient tumor cells exhibited variable but significant decreases in viability, with the exception of Pan02 clone 1-5 ( SI Appendix , Fig. S1 A and B ).

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WebFeb 24, 2010 · The PAN02 murine pancreatic adenocarcinoma cell line was obtained from the National Cancer Institute and maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 100 … sleep down under conferenceWebPan02 Cell 小鼠胰腺癌细胞 BS-C00465757 H1563细胞系；细胞生长特性：贴壁或悬浮，详见细胞说明书部分；细胞形态特性：详见细胞说明书；细胞传代方法：1：2-1：3传代；每周换液2-3次。 DC2.4细胞系；细胞生长特性：贴壁生长 sleep down under 2021 virtual platformWebNov 27, 2024 · D Cell lysates of P and GR PAN02 cells were subjected to Western blot analysis of ERK1/2, phosphorylated ERK1/2, AKT, phosphorylated AKT, and NF-kB. β-Actin was assayed as a loading control. E GR PAN02 cells were infected with OBP-301 or OBP-702 at the indicated MOIs for 72 h. Cell lysates were subjected to Western blot analysis … sleep down meditationWebPanc 02.03 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic … sleep down comforterWebConclusions: Murine Pan_02 cancer model shares many features with human PDAC cancers and therefore it is a good model to study new drugs. Injection of 0.5 × 106 cells … sleep dream pillow okita reviewWebPeter Lassota. (A-D) The 4T1-luc2-C26 and the PC3M-luc-C6 cells were subcutaneously implanted into flank regions of SCID-bg mice. Equal numbers of cells for each cell line was implanted ... sleep dr on kelly and ryanWebPan02 was derived from C57BL/6 mice given orthotopic 3-methyl-cholanthrene and is refractory to many standard chemotherapeutic agents. 1 Pan02 contains a loss of function mutation in the SMAD4 gene that is functionally similar to inactivating mutations in approximately 30% of human pancreatic cancers. 2 Mean and Individual Growth Curves sleep dream pillow australia