WebPoly inosinic:poly cytidylic acid (poly I:C) is a synthetic double-stranded RNA and is a ligand for the Toll like receptor-3. This receptor is involved in the innate immune response to viral infection and poly I:C has been used to mimic the acute phase of a viral infection. The effects of TLR3 activ … WebJan 10, 2024 · Human Embryonic Kidney (HEK) 293T cells were obtained from American Type Culture Collection (ATCC) and were cultured in DMEM supplemented with fetal bovine serum (10%) and penicillin/streptomycin (1%) in 5% CO 2 at 37°C. Poly(I:C) (HMW) was purchased from Invivogen. MG132 and anti-FLAG M2 antibody were from Sigma-Aldrich.
Effects of retinoic acid-inducible gene-I-like receptors activations ...
WebThe present invention relates to compositions comprising polyinosinic (poly(I))-polycytidylic acid poly(C) molecules, or a salt and/or solvate thereof, comprising double-stranded … WebAug 9, 2016 · Poly(I:C) HMW (synthetic double-stranded RNA with high molecular weight) was purchased from InvivoGen (San Diego, CA). IκBα inhibitor Bay11-7082 was purchased from EMD Chemicals (Gibbstown, NJ). Endosomal acidification inhibitor chloroquine (CQ), cycloheximide, and albumin-fluorescein isothiocyanate conjugate (albumin-FITC) were … phobia eye
Poly (I:C)-HMW/LyoVec (100 µg) - Jomar Life Research
WebInvivoGen Pam3CSK4 VacciGrade™ Synthetic triacylated lipopeptide; TLR2/1 agonist. More Info. InvivoGen Poly(I:C) (HMW) VacciGrade™ Polyinosine ... WebTwo different poly(I:C) compounds were used: poly(I:C) with a low molecular weight (LMW, 0.2–1 kb, Invivogen, USA), and poly(I:C) with a high molecular weight (HMW, 1.5–8 kb, … WebFeb 5, 2008 · The ability of poly(I:C) and of LPS to induce a type-I IFN response was assessed by measuring the expression of IFN-β and the ISGs ISG56 and IP-10. poly(I:C) … phobia factory