WebOct 7, 2024 · THP-1 cells (ATCC® TIB202™) were cultured using RPMI-1640 (Life Technologies) supplemented with 2 mM L-glutamine, 10% FCS and Penstrep (100 U/ml) at 37°C, 5% CO 2.Cells were grown to a density of 5 × 10 5 –1 × 10 6 cells/ml and used for experiments between passage 5 and 12. For THP-1 compound treatment and … Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their … See more THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and See more • Cellosaurus entry for THP-1 See more
How to properly freeze and thaw THP1 cells? ResearchGate
WebInvivoGen’s THP-1 cells also respond to NOD1, RIG-I, MDA-5 and STING/cGAS agonists. ... (PMA, catalog #tlrl-pma) induces differentiation of THP1 cells into adherent macrophage-like cells. Day 1 1- Add 180 µl of THP1 cell suspension per well of a 96-well plate (~ 100 000 cells/well). 2- Treat THP1 cells with 20 µl of PMA (final concentration ... WebTHP-1 cell line (ATCC TIB-202) is commonly used to study monocyte and macrophage activities, functions, innate immune mechanisms and signaling pathways. This luciferase reporter cell line was derived from parental line TIB-202 by stably expressing firefly luciferase gene (luc2) under control of a NF-κB promoter. This cell line was established … laptop makes noise
THP-1 Cell-based Assays Monocyte Reporter Cell Lines - InvivoGen
WebCell culture and differentiation The THP-1 cell line was obtained from culture collection of the Chinese Academy of Sciences, Shanghai, China and kept at 1×10^6/ml in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum(FBS) and 1% penicillin-streptomycin (PS). THP-1 cells were Study of the inflammatory cells response to ... WebThe day of transfection, count the cells to determine culture density. Plate 1 x 10 5 cells per well in 0.5 ml of complete growth medium. Cell density should be 50~80% confluent on the day of transfection. For each well of cells to be transfected, dilute 0.5 μg of DNA into 100 μl of Opti-MEM® I Reduced Serum Medium without serum. WebInvivoGen’s THP-1 cells also respond to NOD1, RIG-I, MDA-5 and STING/cGAS agonists. ... (PMA, catalog #tlrl-pma) induces differentiation of THP1 cells into adherent macrophage … laptop me app kaise download